About Workshop
This international workshop introduces participants to Oxford Nanopore sequencing technology and its role in modern biological discovery. The workshop covers nanopore sequencing principles, ONT platforms, sample preparation, library preparation, real-time sequencing, MinKNOW workflows and downstream bioinformatics analysis.
Participants will gain exposure to long-read sequencing applications in genome analysis, transcriptomics, pathogen detection, metagenomics, antimicrobial resistance research and clinical diagnostics. The program is designed for learners who want to understand both the laboratory workflow and computational analysis involved in nanopore-based research.
Aim
To provide participants with a practical and research-oriented understanding of nanopore sequencing technology, from sequencing principles and experimental workflow to genome analysis, metagenomics and clinical applications.
What Participants Will Learn
- Understand the fundamentals of nanopore DNA and RNA sequencing
- Explore the Oxford Nanopore Technology ecosystem, including MinION, Flongle, GridION and PromethION
- Learn sample preparation, quality control, library preparation, barcoding and flow cell workflow concepts
- Understand real-time sequencing, MinKNOW operation and adaptive sampling
- Learn core bioinformatics workflows including basecalling, read QC, mapping, alignment, genome assembly and polishing
- Explore metagenomics and microbial identification using nanopore sequencing data
- Understand applications in pathogen detection, transcriptomics, clinical diagnostics and industry research
Structure
📅 Day 1:Nanopore Sequencing Foundations & Long-Read Genomics
- Evolution of DNA sequencing: Sanger, NGS, Third-Generation Sequencing
- Pangenome and population genomics
- Real-time sequencing and portable genomics
- Hands-on: MinKNOW workflow exploration, ONT platform selection exercise,Public nanopore dataset exploration
📅 Day 2:Sample Preparation, Library Preparation & Sequencing Design
- Experimental design for nanopore projects DNA/RNA extraction and quality assessment High Molecular Weight (HMW)
- DNA preparation workflows
- Run monitoring and troubleshooting
- CRISPR-targeted nanopore sequencing
- Hands-on: Sample QC and library planning
- Barcoding and multiplexing design Sequencing run setup using MinKNOW
📅 Day 3:Nanopore Bioinformatics, AI & Clinical Applications
- POD5/FAST5, FASTQ, BAM/SAM data formats Basecalling with Dorado Read QC, N50, Q-score, coverage analysis
- Structural variant analysis Metagenomics and microbial profiling
- Kraken2 taxonomic classification
- AI-assisted genomics and variant interpretation Cloud and workflow-based bioinformatics (Nextflow, Docker)
- IGV visualization and result interpretation
- Hands-on: Read QC, mapping, and genome visualization
- Genome assembly workflow
- Metagenomic classification using Kraken2
- Methylation analysis workflow
Important Dates
Registration Ends
7:00 PM IST
Workshop Dates
2026-09-02
8:00 PM IST
8:00 PM IST
What You Will Gain
- Live & recorded sessions
- e-Certificate upon completion
- Post-workshop query support
- Hands-on learning experience
Outcomes
- Explain the principle and workflow of nanopore sequencing
- Identify suitable ONT platforms and workflows for different research applications
- Understand sample QC, library preparation, barcoding and sequencing run design
- Interpret nanopore sequencing data outputs such as FASTQ, POD5/FAST5 and BAM/SAM files
- Understand basecalling, quality control, alignment, genome assembly and polishing workflows
- Apply nanopore sequencing concepts to metagenomics, pathogen detection and clinical research use cases
- Design a basic nanopore sequencing-based research workflow for academic or industry projects
Who Should Attend
- PhD scholars and research students
- Professors, faculty members and academicians
- Researchers in biotechnology, genomics, microbiology, molecular biology and life sciences
- Bioinformatics and computational biology learners
- Industry professionals from biotech, pharma, diagnostics, healthcare and agriculture sectors
- International candidates interested in sequencing technologies and genome analysis