Silver Nanoparticle Masterclass: Green Synthesis, Bioengineering & Precision Nanomedicine

This 3-day mentor-led masterclass provides a practical introduction to green-synthesised silver nanoparticles (AgNPs), bioengineering, biomedical applications, and precision nanomedicine. Participants will explore sustainable synthesis, physicochemical characterisation, surface functionalisation, antimicrobial and anticancer activity, toxicity, and targeted delivery concepts. The workshop follows a research-oriented workflow connecting nanoparticle design and characterisation with biological performance, safety, and therapeutic applications.

To provide practical, research-oriented training in green synthesis, characterisation, bioengineering, and biomedical application of silver nanoparticles , with emphasis on therapeutic design, targeted delivery, biological activity, and nanotoxicology.

• Understand the principles and biomedical significance of silver nanoparticles.
• Explore chemical, green, and biogenic synthesis approaches.
• Understand the role of plant extracts, microorganisms, and biomolecules in AgNP synthesis.
• Evaluate synthesis parameters affecting nanoparticle size, morphology, stability, and surface properties.
• Interpret major AgNP characterisation techniques and datasets.
• Understand antimicrobial and anticancer mechanisms of AgNPs.
• Examine ROS generation, oxidative stress, cytotoxicity, and safety considerations.
• Explore protein-corona formation and nanoparticle–biomolecule interactions.
• Understand AgNP surface functionalisation and ligand-based targeting.

📅 Day 1: Green Synthesis, Characterisation & AgNP Design

• Focus: Understand sustainable AgNP synthesis and how physicochemical properties influence biomedical performance.
• Fundamentals of silver nanoparticles and nanomedicine Chemical, green, and biogenic synthesis approaches Plant-, microbial-, and biomolecule-mediated synthesis Role of reducing, capping, and stabilising agents Effects of pH, temperature, precursor concentration, and reaction conditions AgNP size, morphology, crystallinity, surface chemistry, and colloidal stability UV–Vis, FTIR, XRD, SEM/TEM, DLS, and zeta-potential characterisation Structure–property–performance relationships

📅 Day 2: AgNP Bioactivity, Toxicity & Surface Bioengineering

• Focus: Connect AgNP properties with biological activity, toxicity, and functional biomedical design.
• Antimicrobial and antibiofilm mechanisms Anticancer activity, ROS generation, apoptosis, and cellular effects Ag⁺ release and nanoparticle–cell interactions Dose–response and cytotoxicity assessment Hemocompatibility and nanotoxicology considerations Effects of size, charge, coating, and surface chemistry on biological response Protein corona and biomolecular interactions Surface functionalisation and nanoparticle stabilisation PEG, peptides, antibodies, aptamers, and folic-acid targeting concepts

📅 Day 3: Precision Nanomedicine, Targeted Delivery & Candidate Prioritisation

• Focus: Apply AgNP bioengineering concepts to targeted therapeutic delivery and biomedical candidate evaluation.
• AgNPs as therapeutic and drug-delivery platforms Drug loading, surface conjugation, and controlled-release concepts Passive and active targeting strategies Ligand–receptor interactions and targeted cellular uptake Disease-specific and tumour-targeting concepts Optimising size, surface charge, ligand density, drug loading, and stability Integrating efficacy, toxicity, and delivery parameters Computational approaches for nanoparticle performance comparison Multi-parameter candidate ranking Biodistribution, scale-up, reproducibility, and translational considerations Experimental validation and limitations of computational predictions

• Explain AgNP synthesis, properties, and biomedical applications.
• Select appropriate green/biogenic synthesis strategies.
• Interpret key AgNP characterisation data.
• Relate nanoparticle size, morphology, charge, and surface chemistry to biological behaviour.
• Evaluate antimicrobial, anticancer, and cytotoxicity data.
• Understand AgNP-associated ROS, oxidative stress, and safety mechanisms.
• Design surface-functionalised nanoparticles for biomedical applications.
• Differentiate passive and active targeting strategies.
• Develop conceptual AgNP drug-delivery systems.
• Compare nanoparticle formulations using physicochemical and biological parameters.

Graduate and postgraduate students in Nanotechnology, Biotechnology, Biochemistry, Chemistry, Pharmacy, Pharmaceutical Sciences, Bioinformatics, Biomedical Sciences, Microbiology, and Life Sciences. PhD scholars and researchers working in nanomedicine, nanobiotechnology, drug delivery, biomaterials, cancer research, antimicrobial research, and pharmaceutical sciences. Academicians and faculty members interested in green nanotechnology, biomedical nanoparticles, and precision nanomedicine. Industry professionals from pharmaceutical R&D, biotechnology, nanomedicine, drug delivery, formulation development, diagnostics, and biomedical materials.