This 3-day live virtual workshop introduces advanced human-relevant in-vitro models used in disease modeling, drug discovery, toxicology and translational research.
Participants will learn how iPSC-derived models progress from 2D cell systems to spheroids, organoids and dynamic microphysiological/organ-on-chip platforms. Guided hands-on cases will help participants evaluate model quality, analyze experimental data and design reliable follow-up experiments.
Aim
To provide participants with a practical understanding of iPSC, spheroid, organoid, microphysiology and organ-on-chip experimental workflows, with emphasis on experimental design, quality control, validation, troubleshooting and drug-response interpretation.
What Participants Will Learn
Understand iPSC culture, differentiation and quality-control workflows
Design healthy, disease and isogenic experimental groups
Understand spheroid and organoid development and maturation
Evaluate morphology, viability and molecular-marker data
Understand tissue microphysiology, barriers, gradients and mechanical forces
Design MPS and organ-on-chip experiments
Interpret TEER, permeability, viability and biomarker readouts
Identify experimental failures, variability and technical artefacts
Compare 2D, organoid and MPS models for different research applications
Apply reproducibility and validation principles to experimental studies
Structure
Day 1: iPSC Experimental Design & Quality Control
iPSC workflow and research applications
Culture systems, matrices and passaging
Morphology, pluripotency and genomic QC
Healthy, disease and isogenic controls
Replicates, batch effects and experimental endpoints
Common culture failures and troubleshooting
Hands-On: Evaluate iPSC morphology and marker data; design an iPSC disease-model experiment.Tools: Google Colab, microscopy images, iPSC QC dataset
Day 2: Spheroid & Organoid Development and Validation
2D vs spheroid vs organoid models
Spheroid generation and culture parameters
Organoid differentiation, organization and maturation
Tissue-specific organoid models
Morphology, viability and marker-based validation
Variability, reproducibility and experimental failure
Hands-On: Analyze spheroid/organoid images and validation data; classify successful and failed conditions.Tools: Fiji/ImageJ, microscopy dataset, organoid QC data
Day 3: Microphysiology, MPS & Organ-on-Chip Drug Testing
Fundamentals of human tissue microphysiology
Cell–cell, tissue–tissue and microenvironment interactions
Physiological gradients, barriers, mechanical forces and fluid flow
Microphysiological Systems (MPS) and organ-on-chip principles
Chip configuration, perfusion and shear-stress parameters
TEER, permeability, viability and biomarker readouts
Drug efficacy, toxicity and translational applications
MPS validation, troubleshooting and reproducibility
Hands-On: Analyze a virtual MPS/organ-on-chip drug-response experiment and design an optimized study.Tools: Google Colab, Python, pandas, Matplotlib
Important Dates
Registration Ends
4:30 PM
Workshop Dates
2026-08-31
5:00 PM
5:00 PM
What You Will Gain
Outcomes
Design an iPSC-based disease-model experiment
Evaluate iPSC quality and experimental suitability
Assess spheroid and organoid development and validation
Interpret microscopy, viability and marker-expression results
Explain key principles of human tissue microphysiology
Understand flow, shear stress and barrier functions in MPS
Analyze organ-on-chip drug-response datasets
Distinguish biological effects from technical problems
Select appropriate controls, replicates and experimental endpoints
Decide whether an experiment should proceed, be optimized or be repeated
Design an improved follow-up experiment based on the results
Who Should Attend
Undergraduate & Postgraduate Students — Biotechnology, Life Sciences, Biomedical Sciences, Bioengineering, Pharmacy and related disciplines
