CRISPR for Cancer Genomics: A Hands-On Workshop

Cancer genomics and CRISPR-Cas9 technologies are reshaping modern biomedical research. This intensive 3-day workshop provides a unique platform to understand the intersection of these two revolutionary domains. Participants will explore the principles of target gene identification using TCGA/COSMIC databases, design optimized gRNAs with tools like CRISPOR and CHOPCHOP, and address off-target concerns crucial for precision genome editing. The workshop combines theoretical modules with lab-simulated hands-on sessions, covering delivery strategies using viral and non-viral vectors, functional genomic screening with MAGeCK and PinAPL-Py, and post-screening analysis using CRISPResso and IGV. Ethical considerations and data integration practices are also discussed, preparing participants for translational cancer genomics research or industry-oriented roles.

This hands-on workshop aims to equip participants with in-depth knowledge and practical skills to harness CRISPR-Cas9 for cancer research. Through guided tutorials, real-data walkthroughs, and bioinformatics tools, participants will explore gene editing for functional genomics, target validation, and personalized oncology applications.

Day 1:Target Identification and gRNA Design

• Target gene selection (TCGA/COSMIC)
• Off-target prediction and design (CRISPOR, CHOPCHOP)
• Hands-on: Guide RNA design

Day 2: Delivery Systems and Functional Screening

• Viral/non-viral delivery (LentiGuide, AAV)
• Functional screening setup (MAGeCK, PinAPL-Py)
• Hands-on: Troubleshooting delivery

Day 3: Data Analysis and Validation

• Data analysis (MAGeCK, CRISPResso)
• Data integration and ethics (VMD, Integrative Genomics Viewer)
• Hands-on: Data analysis and validation