This 3-day hands-on workshop offers an in-depth exploration of multiplex digital PCR (dPCR)—a cutting-edge technique for absolute quantification of nucleic acids. Participants will learn the principles of dPCR, design multiplex assays, troubleshoot experiments, and analyze real-world data using simulation tools. Applications span infectious disease diagnostics, cancer mutation detection, gene expression analysis, and more. Ideal for molecular biologists, diagnostic researchers, and biotech professionals seeking advanced skills in high-precision PCR technologies.
Aim
The aim of this workshop is to equip participants with a comprehensive understanding of digital PCR (dPCR) technology, with a special emphasis on multiplex assay design, optimization, and application. Through a combination of theoretical instruction, case-based learning, and hands-on simulation, the workshop intends to enable researchers, clinicians, and molecular biologists to leverage multiplex dPCR for precise, absolute quantification of nucleic acids across a range of fields including infectious disease diagnostics, oncology, genetic mutation detection, and GMO testing.
What Participants Will Learn
To introduce the fundamentals of digital PCR and its advantages over qPCR
To explain the principles and applications of multiplex assay design
To train participants in primer/probe design and spectral optimization
To provide hands-on guidance on dPCR instrument operation and data analysis
To demonstrate the application of dPCR in clinical and research settings
To enhance skills in interpreting multiplex results and troubleshooting experiments
