| Attribute | Detail |
|---|---|
| Format | Online, Live + LMS |
| Level | Beginner to Intermediate |
| Duration | 1 Month |
| Certification | e-Certification + e-Marksheet |
| Fee | ₹4999 / $90 |
| Tools | Next-Generation Sequencing FASTQ and FASTA Files SAM and BAM Files VCF Files GTF/GFF Annotation Files Phred Quality Score Read Length, Depth, and Coverage FastQC MultiQC Trimmomatic Cutadapt fastp SAMtools Awareness IGV Genome Browser Awareness Google Colab Linux Terminal Awareness Excel / Google Sheets |
About the NGS Data Processing and Interpretation Course
The NGS Data Processing and Interpretation Internship is designed to introduce learners to the basic concepts of Next-Generation Sequencing and its role in modern biological research. This internship helps participants understand how sequencing data is generated, processed, checked for quality, and interpreted for research applications.
The program is suitable for beginners who want to build a foundation in genomics, bioinformatics, sequencing data formats, quality control, read alignment, variant interpretation, and basic biological reporting. Learners will understand the complete flow of NGS data analysis, starting from raw sequencing reads to meaningful biological interpretation.
Special focus is given to beginner-friendly explanations of NGS file formats, quality scores, read cleaning, mapping statistics, VCF files, RNA-Seq count data, and research report preparation.
Program Highlights
• Beginner-friendly introduction to Next-Generation Sequencing
• Structured understanding of sequencing data and file formats
• Exposure to FASTQ, FASTA, SAM, BAM, VCF, and GTF/GFF files
• Basic understanding of read quality, Phred score, depth, and coverage
• Introduction to quality control using FastQC and MultiQC
• Understanding of read trimming, filtering, and preprocessing concepts
• Basic exposure to alignment, genome mapping, and variant interpretation
• Introduction to RNA-Seq count data and gene expression interpretation
• Focus on simple biological reporting and research summary preparation
• e-Certification + e-Marksheet upon successful completion
Course Curriculum
Module 1: Introduction to NGS
- What is Next-Generation Sequencing?
- Difference between Sanger sequencing and NGS
- Common NGS platforms: Illumina, Ion Torrent, PacBio, Oxford Nanopore
- Applications of NGS in health, agriculture, microbiology, and biotechnology
- Basic types of NGS studies: DNA-Seq, RNA-Seq, WGS, targeted sequencing, and metagenomics
Module 2: Understanding NGS Data
- What are sequencing reads?
- Basic NGS file formats: FASTQ, FASTA, SAM, BAM, VCF, GTF/GFF
- Introduction to read quality
- Phred quality score in simple terms
- Read length, depth, and coverage
- Why good-quality data is important
Module 3: Quality Check and Data Cleaning
- Why quality control is needed in NGS
- Introduction to FastQC reports
- Common quality issues in sequencing data
- Adapter contamination and low-quality reads
- Basics of read trimming and filtering
- Introduction to tools like FastQC, MultiQC, Trimmomatic, Cutadapt, and fastp
Module 4: Basic NGS Analysis and Reporting
- Overview of a simple NGS analysis workflow
- Introduction to read alignment and genome mapping
- Basic idea of SAM/BAM files
- Introduction to variant calling and VCF files
- Basic idea of RNA-Seq count data and gene expression
- Introduction to biological interpretation
- Preparing a simple NGS analysis summary report
Tools, Techniques, or Platforms Covered
Next-Generation Sequencing FASTQ and FASTA Files SAM and BAM Files VCF Files GTF/GFF Annotation Files Phred Quality Score Read Length, Depth, and Coverage FastQC MultiQC Trimmomatic Cutadapt fastp SAMtools Awareness IGV Genome Browser Awareness Google Colab Linux Terminal Awareness Excel / Google Sheets
Real-World Applications
- Understanding raw sequencing data generated from NGS platforms
- Checking sequencing read quality before analysis
- Identifying adapter contamination and low-quality reads
- Interpreting basic QC reports for research use
- Understanding alignment statistics from mapped sequencing reads
- Reading and interpreting basic VCF files
- Understanding RNA-Seq count data and gene expression results
- Preparing simple NGS data interpretation summaries
- Supporting genomics, transcriptomics, microbiology, agriculture, biotechnology, and clinical research workflows
Who Should Attend & Prerequisites
- This internship is suitable for students, researchers, PhD scholars, faculty members, laboratory professionals, biotechnology learners, bioinformatics beginners, life science learners, and professionals interested in NGS data analysis and interpretation.
- It is useful for learners from biotechnology, genetics, genomics, microbiology, molecular biology, biomedical science, bioinformatics, agriculture, clinical research, and related fields.
Certification
