| Attribute | Detail |
|---|---|
| Format | Recorded Lectures |
| Level | Intermediate |
| Duration | 3 Days (60-90 minutes) |
| Certification | e-Certification + e-Marksheet |
| Fee | Free |
| Tools | FastQC MultiQC fastp Trimmomatic SAMtools IGV Google Colab Google Sheets FASTQ Viewer |
About the Hands-on Introduction to NGS Data Analysis: From Raw Sequencing Reads to Biological Interpretation Course
This 3‑day beginner‑friendly program introduces the fundamentals of Next‑Generation Sequencing (NGS) data processing and biological interpretation.
Learners will explore raw FASTQ files, perform quality control, clean reads, and gain a practical overview of alignment, variant calling and RNA‑Seq interpretation using live demos and hands‑on activities.
Program Highlights
• Comprehensive coverage of Hands from fundamentals to advanced applications
• Hands-on projects and real-world case studies in biotechnology
• Expert-curated curriculum aligned with current industry standards
• Access to recorded lectures and e-LMS platform for flexible, self-paced learning
• e-Certification and e-Marksheet upon successful completion
• Dedicated mentor support and interactive doubt-clearing sessions
• Practical experience with tools: FastQC, MultiQC, fastp, Trimmomatic
• Career-oriented training for academic and professional growth in biotechnology
Course Curriculum
Module 1: Day 1 – Introduction to NGS and Sequencing Data Fundamentals
- Explore major NGS platforms and applications
- Identify raw sequencing reads and file formats (FASTQ, SAM, VCF…)
- Interpret Phred scores, read length, depth and coverage
- Navigate a standard NGS analysis pipeline
Module 2: Day 2 – Quality Control, Read Cleaning and Preprocessing
- Run FastQC and generate MultiQC summary reports
- Detect adapters, low‑quality bases and duplication
- Trim and filter reads using fastp / Trimmomatic
- Compare raw vs cleaned reads and document QC metrics
Module 3: Day 3 – Basic NGS Workflow Interpretation & Biological Reporting
- Review alignment concepts and SAM/BAM statistics
- Explore variant calling basics and VCF interpretation
- Introduce RNA‑Seq count data and differential expression overview
- Visualise results with IGV and simple plotting
Tools, Techniques, or Platforms Covered
FastQC MultiQC fastp Trimmomatic SAMtools IGV Google Colab Google Sheets FASTQ Viewer
Real-World Applications
- Apply Hands skills directly to academic research, thesis work, and publications
- Build a professional portfolio showcasing practical biotechnology competencies
- Solve industry-relevant problems using Hands methodologies and tools
- Contribute to open-source projects and collaborative research in biotechnology
- Prepare for competitive examinations, interviews, and professional certifications in biotechnology
Who Should Attend & Prerequisites
- Students pursuing degrees in biotechnology, science, engineering, or related disciplines
- Working professionals seeking to upskill or transition into biotechnology roles
- Researchers and academicians looking to adopt modern techniques in biotechnology
- Entrepreneurs, freelancers, and self-learners interested in practical biotechnology knowledge
Certification
