| Attribute | Detail |
|---|---|
| Format | Online, Live + LMS |
| Level | Beginner |
| Duration | 1 Month |
| Certification | e-Certification + e-Marksheet |
| Fee | ₹2999 / $59 |
| Tools | NCBI GenBank BLAST UniProt Ensembl Plants Gramene KEGG Plant Reactome Google Scholar PubMed Google Colab Python Excel Google Sheets |
About the Development of C4 Plants Using rDNA Technology Course
Attribute
Detail
Format
Online, Live + LMS
Level
Beginner to Intermediate
Duration
1 Month
Certification
e-Certification + e-Marksheet
Category
Internship
Tools
NCBI, BLAST, GenBank, UniProt, Ensembl Plants, KEGG, Google Colab, Python, Excel / Google Sheets
The Development of C4 Plants Using rDNA Technology Internship introduces learners to C3 and C4 photosynthesis, recombinant DNA technology, plant genetic engineering, gene regulation, and crop improvement.
Participants will understand how C4-related genes and pathways can be introduced into C3 crops such as rice and wheat to improve photosynthetic efficiency, water-use efficiency, productivity, and climate resilience.
The internship includes guided activities in gene identification, biological database searching, conceptual vector design, plant transformation workflows, CRISPR awareness, biosafety, and research-report preparation.
Program Highlights
• Introduction to C3 and C4 photosynthesis
• Understanding of C4 genes, enzymes, and pathways
• Basics of recombinant DNA technology
• Plant-vector and promoter-selection concepts
• Exposure to plant transformation and gene stacking
• Case studies on C4 rice and wheat
• Introduction to CRISPR and synthetic biology
• Use of plant genomics databases
• Research proposal and report preparation
• e-Certification + e-Marksheet
Course Curriculum
Module 1: C3 and C4 Photosynthesis
- Basics of photosynthesis and carbon fixation
- C3, C4, and CAM pathways
- Photorespiration
- Kranz anatomy
- Mesophyll and bundle-sheath cells
- Water-use and nitrogen-use efficiency
- Importance of C4 engineering in crops
Module 2: Molecular and Genetic Framework
- Major C4 genes and enzymes
- PEPC, PPDK, NADP-malic enzyme, and carbonic anhydrase
- RuBisCO and RuBisCO activase
- Promoters, terminators, and selectable markers
- Gene and protein sequence searching
- NCBI, BLAST, GenBank, and UniProt
Module 3: rDNA Technology and Plant Transformation
- Gene cloning and recombinant DNA concepts
- Plant-expression vectors
- Binary vectors and Ti plasmids
- Gene stacking and multigene expression
- Agrobacterium-mediated transformation
- Particle bombardment awareness
- PCR and gene-expression validation
- Conceptual vector-design activity
Module 4: C4 Crop Engineering and Biosafety
- Engineering C4 traits in C3 crops
- C4 rice and wheat case studies
- CRISPR-Cas and synthetic biology
- Biosafety and environmental risk
- GMO regulations and field trials
- Research proposal and internship-report preparation
Tools, Techniques, or Platforms Covered
NCBI GenBank BLAST UniProt Ensembl Plants Gramene KEGG Plant Reactome Google Scholar PubMed Google Colab Python Excel Google Sheets
Real-World Applications
- Identification of C4 genes and enzymes
- Comparison of C3 and C4 pathways
- Plant gene and protein sequence analysis
- Conceptual plant-vector design
- Crop-transformation workflow development
- CRISPR-based crop-improvement awareness
- Biosafety and GMO assessment
- Plant biotechnology research and thesis support
- Climate-resilient crop-development studies
Who Should Attend & Prerequisites
- Biotechnology, botany, agriculture, and plant science students
- Genetics, genomics, molecular biology, and biochemistry learners
- Undergraduate and postgraduate students
- PhD scholars and researchers
- Faculty members
- Plant-breeding and tissue-culture professionals
- Bioinformatics beginners
- Learners interested in crop biotechnology and genetic engineering
Certification
