Home /Artificial Intelligence /Course /Basics of High-Throughput Sequencing

Basics of High-Throughput Sequencing

AttributeDetail
FormatOnline, self-paced course
LevelBasic / Beginner
Duration2–3 Weeks
Certificatione-Certification
Fee₹199 / $20
ToolsHigh-Throughput Sequencing Next-Generation Sequencing (NGS) Genomic Data RNA-Seq DNA Sequencing

About the Basics of High-Throughput Sequencing Course

The Basics of High-Throughput Sequencing course is a free, beginner-friendly self-paced program designed to introduce learners to the technologies and techniques used in high-throughput sequencing (HTS), also known as next-generation sequencing (NGS).

The course explains the fundamentals of sequencing technologies used to generate vast amounts of genetic data, including DNA and RNA sequencing. Learners will explore how HTS is applied in genomics, disease research, agriculture, and biotechnology, and how it enables large-scale analysis of genes, genomes, and epigenomes.

Program Highlights

• Free beginner-level HTS course

• Online self-paced learning format

• Simple explanation of sequencing technologies and data

• Covers sequencing platforms, data generation, and analysis basics

• Real-world examples from genomics, healthcare, and research

• Suitable for students and non-technical learners

• e-Certification upon successful completion

Course Curriculum

Module 1: Introduction to High-Throughput Sequencing

  • What is High-Throughput Sequencing (HTS)?
  • Overview of Next-Generation Sequencing (NGS)
  • Applications in Genomics, Transcriptomics, and Epigenomics
  • Importance of HTS in Modern Biological Research

Module 2: Sequencing Technologies and Platforms

  • Different Types of Sequencing Technologies (e.g., Illumina, PacBio, Oxford Nanopore)
  • How HTS Works: From Sample Preparation to Sequencing
  • Understanding Read Length and Coverage
  • Choosing the Right Sequencing Platform for Your Research

Module 3: Data Generation and Processing

  • Sequencing Data Formats (FASTQ, BAM, VCF)
  • Quality Control of Sequencing Data
  • Introduction to Data Alignment and Mapping
  • Understanding Sequencing Depth and Accuracy

Module 4: Applications of High-Throughput Sequencing

  • Whole Genome Sequencing and Variant Detection
  • RNA-Seq and Transcriptomic Analysis
  • Metagenomics and Microbiome Analysis
  • Applications in Disease Research, Drug Discovery, and Agriculture

Module 5: Future Scope and Learning Path

  • Emerging Trends in HTS and Single-Cell Sequencing
  • Integration with AI and Big Data in Genomics
  • Career Opportunities in Genomics and Biotechnology
  • Mini Learning Activity / Concept-Based Practice

Tools, Techniques, or Platforms Covered

High-Throughput Sequencing Next-Generation Sequencing (NGS) Genomic Data RNA-Seq DNA Sequencing

Real-World Applications

  • Understanding genetic variation and mutations
  • Analyzing gene expression using RNA-Seq
  • Identifying microbial communities through metagenomics
  • Supporting personalized medicine and drug discovery
  • Exploring large-scale genomic data in research and industry

Who Should Attend & Prerequisites

  • This course is suitable for students, beginners, researchers, and professionals interested in genomics, biotechnology, and sequencing technologies.
  • It is also useful for learners from biotechnology, bioinformatics, genetics, molecular biology, medicine, pharmacy, biomedical science, and research backgrounds.
Prerequisites: No prior HTS or sequencing knowledge is required. Basic understanding of biology or interest in genomics is helpful but not mandatory.

Certification

Sample certificate
Hi! Need help? Chat with NSTC ✨